mitocheck® mitochondrial oxygen consumption rate (ocr) assay kit Search Results


ocr plate assay kit  (Dojindo Labs)
95
Dojindo Labs ocr plate assay kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Ocr Plate Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/Extracellular+OCR+Plate+Assay+Kit/pm40002369-68-6-10
Average 95 stars, based on 1 article reviews
ocr plate assay kit - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
finereader engine  (ABBYY USA Software House Inc)
90
ABBYY USA Software House Inc finereader engine
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Finereader Engine, supplied by ABBYY USA Software House Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/finereader+engine/us10885106-2475-12-18
Average 90 stars, based on 1 article reviews
finereader engine - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rneasy miniprep kit  (Qiagen)
99
Qiagen rneasy miniprep kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Rneasy Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/RNeasy+Plus+Mini+Kit/pm39799584-459-54-57
Average 99 stars, based on 1 article reviews
rneasy miniprep kit - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
asprise ocr software development kit (sdk)  (ABBYY USA Software House Inc)
90
ABBYY USA Software House Inc asprise ocr software development kit (sdk)
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Asprise Ocr Software Development Kit (Sdk), supplied by ABBYY USA Software House Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/asprise+ocr+software+development+kit++sdk+/us11036695-1166-12-18
Average 90 stars, based on 1 article reviews
asprise ocr software development kit (sdk) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
ppd49 26  (Addgene inc)
93
Addgene inc ppd49 26
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Ppd49 26, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/Fire+Lab+C%2E+elegans+Vector+Kit+(Kit+%231000000001)/pmc03177883-119-40-47
Average 93 stars, based on 1 article reviews
ppd49 26 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
oracollect • dna kit (ocr-100)  (DNA Genotek)
90
DNA Genotek oracollect • dna kit (ocr-100)
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Oracollect • Dna Kit (Ocr 100), supplied by DNA Genotek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/liquid+based+kit+oracollect+dna+ocr+100/nct04832126-79-7-12
Average 90 stars, based on 1 article reviews
oracollect • dna kit (ocr-100) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
oragene ocr-100 saliva kit  (DNA Genotek)
90
DNA Genotek oragene ocr-100 saliva kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Oragene Ocr 100 Saliva Kit, supplied by DNA Genotek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/oragene+kits/pm39435968-56-7-12
Average 90 stars, based on 1 article reviews
oragene ocr-100 saliva kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
mito-id extracellular o 2 sensor kit  (Enzo Biochem)
90
Enzo Biochem mito-id extracellular o 2 sensor kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Mito Id Extracellular O 2 Sensor Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/mito+id++extracellular+o2+sensor+kit++high+sensitivity/pmc06803469-86-8-16
Average 90 stars, based on 1 article reviews
mito-id extracellular o 2 sensor kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
ppd95 77  (Addgene inc)
93
Addgene inc ppd95 77
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Ppd95 77, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/pPD95_77+(Plasmid+%231495)/pmc03177883-119-56-63
Average 93 stars, based on 1 article reviews
ppd95 77 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
e myco plus, mycoplasma ocr detection kit  (iNtRON Biotechnology)
90
iNtRON Biotechnology e myco plus, mycoplasma ocr detection kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
E Myco Plus, Mycoplasma Ocr Detection Kit, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/e+myco+mycoplasma+pcr+detection+kit/pmc07004527-92-13-17
Average 90 stars, based on 1 article reviews
e myco plus, mycoplasma ocr detection kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
advantage kit  (Quanterix)
96
Quanterix advantage kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Advantage Kit, supplied by Quanterix, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/NF-Light+v2+Advantage+Kit/10__1177_slash_1352458519868070-1610-8-7
Average 96 stars, based on 1 article reviews
advantage kit - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
cs assay kit  (Merck KGaA)
90
Merck KGaA cs assay kit
Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal <t>oxygen</t> <t>consumption</t> <t>rate</t> <t>(OCR)</t> in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.
Cs Assay Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitocheck%C2%AE+mitochondrial+oxygen+consumption+rate+(ocr)+assay+kit/cs+assay+kit/pmc09643307-45-12-15
Average 90 stars, based on 1 article reviews
cs assay kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal oxygen consumption rate (OCR) in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.

Journal: Antioxidants (Basel, Switzerland)

Article Title: The Effects of Fisetin on Gene Expression Profile and Cellular Metabolism in IFN-γ-Stimulated Macrophage Inflammation.

doi: 10.3390/antiox14020182

Figure Lengend Snippet: Figure 3. Fisetin modulated metabolism in IFN-γ-stimulated macrophages. (A,B) GSEA plot of oxidative phosphorylation (OXPHOS) gene set in IFN-γ-stimulated cells compared to non-treated cells (A) and in fisetin-pretreated IFN-γ-stimulated cells compared to IFN-γ-stimulated cells (B). The gene set was from mouse-ortholog hallmark gene sets of the molecular signatures database. (C) Expression heatmap of representative genes from OXPHOS gene set. Color gradient reflects row Z-score. (D) Fisetin rescued impaired basal oxygen consumption rate (OCR) in IFN-γ-induced macrophages (n = 6). (E) Fisetin decreased IFN-γ-enhanced lactate production (n = 3). For (D,E), RAW264 cells were pre-cultured for 21 h and starved in serum-free medium for 2.5 h. The cells were then treated with 0–5 µM fisetin for 30 min and subsequently exposed to 10 ng/mL IFN-γ for 12 h. The cells were used for OCR measurement, and the culture medium was collected for lactate determination. Each value represents the mean ± SD; different letters between groups indicate significant differences (p < 0.05). For OCR and lactate determination, at least two separate experiments were performed.

Article Snippet: OCR was determined with an extracellular OCR plate assay kit (Dojindo, Mashiki, Japan) according to the manufacturer’s manual.

Techniques: Phospho-proteomics, Expressing, Cell Culture